![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3086009:Design
Designed by: Nikila Ojili Group: iGEM19_Florida (2019-10-18)
SCRIBE (RpoB) System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1877
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1877
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1282
Illegal XhoI site found at 516 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1877
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1877
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Changed msDNA target to rpoB to screen for rifampicin resistance
Source
PFF745 plasmid from lab of Prof. Tim Lu (iGEM registry Bba_K1681000)