Composite

Part:BBa_K3086009:Design

Designed by: Nikila Ojili   Group: iGEM19_Florida   (2019-10-18)


SCRIBE (RpoB) System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1282
    Illegal XhoI site found at 516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1877
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changed msDNA target to rpoB to screen for rifampicin resistance


Source

PFF745 plasmid from lab of Prof. Tim Lu (iGEM registry Bba_K1681000)

References